mouse kat2a gcn5  (Addgene inc)

Journal: Nature communications

Article Title: The WHHERE coactivator complex is required for retinoic acid-dependent regulation of embryonic symmetry.

doi: 10.1038/s41467-017-00593-6

Figure Lengend Snippet: Fig. 7 Kat2a but not Ep300 acts as a coactivator for retinoic acid signaling. a–e RARE-Luciferase activity from NIH3T3 cells treated or not with 1 µM RA for 20 h. a Cells transfected with expression plasmids containing Ep300, Hdac1, or both (n = 4). b Cells transfected with expression plasmids containing human Hdac1 (H1-WT) or Hdac1 mutant (H1-6R) (n = 4). c Cells transfected with expression plasmids containing Rarα, Rarα, and Ep300 or Rarα and Kat2a (n = 3). d Cells transfected with expression plasmids containing Rere, Rere and Ep300 or Rere and Kat2a (n = 3). e Cells treated either with siRNA for Rere, Ep300, or Kat2a (n = 4). In all graphs data represent mean ± s.e.m. NS—not significant, *P < 0.05 and **P < 0.01. Student’s unpaired two-tailed t-test. f, g RARE-LacZ expression in wild-type Ep300+/+ f and Ep300–/– g embryos at E8.75-E9.0 (dorsal views). h, i In situ hybridization showing somites labeled with Uncx4.1 in wild-type Ep300+/+ h and Ep300–/– i embryos at E8.75-E9.0 (dorsal views). For each genotype at least 5–10 embryos were analyzed. Bar = 100 microns

Article Snippet: Human Ep300 in pCMVb was a gift from Richard Eckner and mouse Kat2a (Gcn5) in pCMV-sport247 (Invitrogen) (Addgene plasmid 23098) was a gift from Sharon Dent.

Techniques: Luciferase, Activity Assay, Transfection, Expressing, Mutagenesis, Two Tailed Test, In Situ Hybridization, Labeling

Journal: Cell cycle (Georgetown, Tex.)

Article Title: Single nucleosome ChIPs identify an extensive switch of acetyl marks on cell cycle promoters.

doi: 10.4161/cc.9.11.11839

Figure Lengend Snippet: Figure 6. Effect of KAT2A inactivation in KAT2B-/- MEFs. (A) Western blot analysis of nuclear extracts KAT2B wt, KAT2B-/- MEFs with the antibodies indicated and normalized with Lamin B. (B) Western blot analysis of acid extracts of KAT2B wt and KAT2B-/- MEFs, transfected with siRNA for KAT2A or control scramble siRNA, prepared at three different time points (24, 48, 72 hour post-transfection). The antibodies used are indicated. The H3 antibody was used as loading control and for normalization. (C) Flow citometry histograms of KAT2B wt and KAT2B-/- MEFs, transfected with siRNA for KAT2A or control scramble siRNA at three different time points (24, 48, 72 h). Percentage of cells in the different phases of the cell cycle are shown. (D) Gene expression analysis of cell cycle genes of KAT2B wt and KAT2B-/- MEFs, transfected with siRNA for KAT2A or control scramble siRNA; gene expression was analyzed by q-PCR with the appropriate primers and GAPDH was used for normalization.

Article Snippet: The KAT2A siRNA (mouse) was purchased by Santa Cruz Biotechnology (sc-37947).

Techniques: Western Blot, Transfection, Control, Gene Expression